Zone of Inhibition Tests

Test Purpose
The purpose of this microbiology lab test is to evaluate the basic antibacterial activity of an agent against selected bacteria by determining the zone of inhibition. This test is typically used for development purposes.

Test Summary
An agar plate is inoculated with a lawn of a bacterial culture suspension. The test material is placed in contact with the inoculated surface. Following incubation, the agar surface is examined for inhibition of test organism growth. Appropriate purity and sterility controls are performed.

Topical Antimicrobial tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  AOAC Available Chlorine Equivalency Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy on Surrogate Skin Surfaces (Ex-Vivo)

l  Zone of Inhibition Tests