Virucidal Efficacy Tests
Test Purpose
The purpose of this test is to
evaluate the virucidal efficacy
of a client's liquid or spray
product against a specific virus
according to test criteria and
methods approved by the U.S.
Environmental Protection Agency
for registration of a product as
a virucide for hard, non-porous
surfaces. The AOAC Use Dilution
for Liquids microbiology lab
test or the AOAC Germicidal
Spray microbiology lab test must
be conducted prior to running
the virucidal efficacy test.
Test Summary
A film of virus, dried on a
glass surface, is exposed to the
test substance for a specified
exposure time. Following
exposure, the virucidal and
cytotoxic activities are removed
from the virus-test substance
mixture, and the mixture is
assayed for viral infectivity by
an accepted assay method.
Appropriate virus, test
substance cytotoxicity, and
neutralization controls are run
concurrently.
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