Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Tests

Test Purpose
The purpose of this microbiology test is to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of a test material against selected microorganisms using a Macrodilution broth method to comply with the U.S. Environmental Protection Agency’s requirements.

Test Summary
The test material is diluted in a series of two-fold dilutions in test tubes. A suspension of each test organism is added to the test tube containing the test material dilution. The test tubes are incubated, and observed for visible growth. After observation, the test tubes demonstrating no visible turbidity are subcultured to solid agar plates and the MIC and MBC are determined.

Typical microbiology laboratory product development/screening tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy Tests

l  AOAC Use Dilution Test

l  AOAC Germicidal Spray Products Test

l  ASTM Pre-Saturated Towelettes Test

l  ASTM Non-Food Contact Sanitizer Test

l  AOAC Germicidal and Detergent Sanitizer Test

l  AOAC Tuberculocidal Activity Test

l  AOAC Sporicidal Activity Test