Food Contact Sanitizer Testing
for Towelettes
Test Purpose
The purpose of this microbiology
lab test is to quantitatively
evaluate the antimicrobial
efficacy of a client's
presaturated towelette sanitizer
product for use on pre-cleaned,
nonporous food contact surfaces
to comply with the U.S.
Environmental Protection
Agency’s requirements. The
product must demonstrate a
≥99.999% reduction of bacteria
in 30 seconds. This method is
based on EPA’s "Draft Interim
Guidance for Non-Residual
Sanitization of Hard Inanimate
Food Contact Surfaces Using
Pre-Saturated Towelettes"
published on April 12, 2001.
Test Summary
A film of bacterial cells dried
on a surface of large glass and
plastic carriers totaling 4 ft2
is exposed to the sanitizer for
a 30 second contact time. After
exposure, the carriers are
processed with neutralizing
subculture media and assayed
quantitatively for survivors.
Appropriate viability,
sterility, purity, carrier
quantitation and neutralization
controls are performed.
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