EN 14476 Quantitative Suspension
Method for Virucidal Activity
Test Purpose
The purpose of this test is to
determine the minimum virucidal
activity of a chemical
disinfectant in contact with
medical instruments, surfaces or
hands, according to the test
method EN 14476 against relevant
viruses. The method described is
intended to determine the
virucidal activity of chemical
disinfectants on viruses in the
conditions in which they are
used.
Test Summary
A suspension of virus is added
to a solution of interfering
substances. A prepared test
solution of a product is added
to the suspension of virus in
interfering substances. The
mixture is maintained at the
requested temperature
for the requested exposure time(s). At the end of the
exposure time, aliquots are
taken and the virucidal activity
against the test virus in this
portion is immediately
neutralized or suppressed. The
number of surviving viral
particles in the test suspension
are determined by serial
dilution. Appropriate controls
are also performed.
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