AOAC Sporicidal Activity Test

Test Purpose
The purpose of this microbiology lab test is to determine the sporicidal activity of a germicide against specified spore-forming bacteria using the AOAC Sporicidal Activity Method. This test is used to substantiate sporicidal and sterilant claims to comply with the U.S. Environmental Protection Agency and other regulatory agency requirements.

Test Summary
A suspension of bacterial spores dried on specified carriers (porcelain penicylinders and silk or Dacron sutures) is exposed to the test substance for a specified contact time. After exposure, the carriers are transferred to vessels containing neutralizing subculture media and assayed for survivors. Appropriate HCl, viability, carrier quantitation and neutralization controls are performed.

Typical microbiology laboratory product development/screening tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy Tests

l  AOAC Use Dilution Test

l  AOAC Germicidal Spray Products Test

l  ASTM Pre-Saturated Towelettes Test

l  ASTM Non-Food Contact Sanitizer Test

l  AOAC Germicidal and Detergent Sanitizer Test

l  AOAC Tuberculocidal Activity Test

l  AOAC Sporicidal Activity Test