AOAC Germicidal and Detergent
Sanitizer Tests
Test Purpose
The purpose of this microbiology
lab test is to quantitatively
evaluate the antimicrobial
efficacy of a client's sanitizer
product for use on pre-cleaned,
nonporous food contact surfaces
to comply with the U.S.
Environmental Protection
Agency’s requirements. The
product must demonstrate a
≥99.999% reduction in 30
seconds.
Test Summary
A suspension of bacterial cells
is exposed to the sanitizer for
a specified contact time (30
seconds). After exposure, an
aliquot of the exposed
suspension is transferred to
vessels containing neutralizing
subculture media and assayed
quantitatively for survivors.
Appropriate numbers control,
purity, sterility, viability,
and neutralization controls are
performed.
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