AOAC Confirmatory Tuberculocidal
Activity Test
Test Purpose
The purpose of this microbiology
lab test is to evaluate the
tuberculocidal effectiveness of
a client's product against
Mycobacterium bovis (BCG), an
acceptable surrogate for
Mycobacterium tuberculosis,
following the AOAC Tuberculocidal Activity Test, to
comply with the U.S.
Environmental Protection Agency
and other regulatory agency
requirements.
Test Summary
A film of mycobacterial cells
dried on porcelain penicylinder
carriers is exposed to the test
substance for a specified
contact time. After exposure,
the carriers are transferred to
vessels containing neutralizing
subculture media and assayed
qualitatively for survivors.
Appropriate viability, carrier
population, and neutralization
controls are performed.
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