AOAC Confirmatory Tuberculocidal Activity Test

Test Purpose
The purpose of this microbiology lab test is to evaluate the tuberculocidal effectiveness of a client's product against Mycobacterium bovis (BCG), an acceptable surrogate for Mycobacterium tuberculosis, following the AOAC Tuberculocidal Activity Test, to comply with the U.S. Environmental Protection Agency and other regulatory agency requirements.

Test Summary
A film of mycobacterial cells dried on porcelain penicylinder carriers is exposed to the test substance for a specified contact time. After exposure, the carriers are transferred to vessels containing neutralizing subculture media and assayed qualitatively for survivors. Appropriate viability, carrier population, and neutralization controls are performed.

Typical microbiology laboratory product development/screening tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy Tests

l  AOAC Use Dilution Test

l  AOAC Germicidal Spray Products Test

l  ASTM Pre-Saturated Towelettes Test

l  ASTM Non-Food Contact Sanitizer Test

l  AOAC Germicidal and Detergent Sanitizer Test

l  AOAC Tuberculocidal Activity Test

l  AOAC Sporicidal Activity Test